Analysis of microbial populations in plastic–soil systems after exposure to high poly(butylene succinate-co-adipate) load using high-resolution molecular technique

Abstract Background Bio-based and biodegradable plastics are considered as of the future owing to their ability decompose under various environmental conditions. However, effects on soil microbiome poorly characterised. In this study, we aimed investigate an important bio-based plastic, polybutylene succinate-co-adipate (PBSA), microbial diversity community composition using high-resolution molecular technique (Illumina sequencing) targeting all three domains: archaea, bacteria, fungi. Results Adding high load PBSA (6% (w/w)) caused a significant decline in archaeal (13%) fungal (45%) richness substantial changes both bacterial (Proteobacteria, Actinobacteria, Acidobacteria) (Eurotiomycetes, Sordariomycetes, Leotiomycetes, Dothideomycetes) compared with no addition soil. The combined (NH 4 ) 2 SO fertilisation were much greater than alone. We only detected opportunistic human pathogens low abundance surrounding some plant pathogenic fungi and/or enriched films Apart from pathogens, many potential control agents growth-promoting microorganisms also detected/enriched addition. together can either eliminate or enrich specific especially Fusarium solani , which is economically important. Conclusions conclude that plastic may negatively affect microbiome.